Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (46): 8018-8023.doi: 10.3969/j.issn.2095-4344.2013.46.007
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Zhang Yu-qiang1, Li You2, Cao Yang1, Dong Ming-yan1, Lü Gang1
Online:
2013-11-12
Published:
2013-11-30
About author:
Zhang Yu-qiang☆, M.D., Department of Orthopedics, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China
zhangyuqiang1981@163.com
CLC Number:
Zhang Yu-qiang, Li You, Cao Yang, Dong Ming-yan, Lü Gang. Effect of hyperbaric oxygen preconditioning on expression of Bcl-2 and Bax after spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(46): 8018-8023.
2.1 实验动物数量分析 纳入SD大鼠66只,其中7只大鼠因术后感染死亡,并进行了补充,在进入结果分析时无脱失。 2.2 高压氧预处理对大鼠损伤脊髓中Bcl-2表达的影响 免疫组化染色显示,Bcl-2阳性表达的特征为神经元和胶质细胞胞浆或/和胞膜见棕黄色颗粒。假手术组脊髓组织中可见少量Bcl-2阳性细胞,见图1A。模型组脊髓灰质和白质中均可见Bcl-2阳性细胞,脊髓损伤8 h仅有少量Bcl-2阳性细胞,损伤第1天时Bcl-2阳性细胞开始增多,损伤第3天Bcl-2阳性细胞数进一步增加,损伤第7天达高峰,损伤后第14天仍可见大量Bcl-2阳性细胞,见图1B。高压氧预处理组脊髓灰质和白质均表达Bcl-2,表达高峰亦出现在伤后第7天,但高压氧预处理组大鼠脊髓中Bcl-2阳性细胞数量均显著高于模型组(P < 0.05),见图1C,表1。"
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